Cyklodextrin Komplex Struktur - HPBCD - Nyheter - Shandong

Cloning and expression of a putative cyclodextrine

CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are extensively used in agriculture, chemicals, cosmetics, foods and pharmaceuticals . Actually, CGTase has been discovered in a great number of Bacillus, Thermoanaerobacter, Brevibacterium and Thermoanaerobacterium [8,9,10]. Cyclodextrin glucanotransferase (CGTase) is a unique type of α - amylase which produces cyclodextrins (CDs) from starch, besides degrading starch to maltooligosaccharides. The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes cyclic malto-oligosaccharides called cyclodextrins (CDs) from starch and related α-1,4-glucans. CGTases are produced by a variety of bacteria, mainly Bacillus species, by submerged culture in complex medium. CGTases differ in the amount and types of CDs produced.

Cgtase

Paenibacillus Sp A11, CLIPtechnique. Stipendiat. 24B Korpraditskul,. Prof. Dr. Vichai.

Cloning and expression of a putative cyclodextrine

Anaerobranca gottschalkii. Status.

Ngoc Trang Nhu Ngo - Lund, Sverige Professionell profil

The effects of buffer ion strength and pH, and enzyme loading on … Information on EC 2.4.1.19 - cyclomaltodextrin glucanotransferase. cyclomaltodextrins (Schardinger dextrins) of various sizes (6,7,8, etc. glucose units) are formed … Cyclodextrin glycosyltranferase (CGTase EC 2.4.1.19) is a bacterial enzyme that converts starch and other 1,4-linked α-glucans to cyclodextrins (closed-ring structures composed mainly of 6, 7 and 8 glucosyl units, named α, β and γ-cyclodextrin, respectively) (French, 1957; Thoma and Stewart, 1965). Cyclodextrins have the ability to form inclu- The gene encoding the cyclodextrin glycosyltransferase (CGTase) of Paenibacillus pabuli US132, previously described as efficient antistaling agent and good candidate for cyclodextrins production, was cloned, sequenced, and expressed in Escherichia coli .

Status.
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Cyclodextrin glycosyltransferase (CGTase) is an enzyme of the alpha-amylase family, which uses a double displacement mechanism to process alpha-linked glucose polymers. We have determined two X-ray structures of CGTase complexes, one with an intact substrate at 2.1 A resolution, and the other with a covalently bound reaction intermediate at 1.8 A resolution. CGTase enzyme production is depicted in Figs 2 and 3 for Bacillus sp. and B. circulance, respectively. The CGTase activity in culture broths was observed increasing with increase in initial inoculum level for Fig. 1⎯CGTase production pattern in Bacillus sp.

We have determined the 2.0 and 2.5 Å X-ray structures of E257A/D229A CGTase in complex with The CGTase residue has a C-alpha atom located < 6 Å from a heavy atom (i.e., an atom other than H) of an amino acid residue of the maltogenic alpha-amylase corresponding to residue 190-194 of SEQ ID NO: 17.
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rekombinant - Deutsch-Übersetzung – Linguee Wörterbuch

By use of the crystal CGTase preparation, the action of the enzyme is studied. Purpose γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. However, the low specificity and activity of soluble γ-CGTase increase the production cost of γ-CD, thereby limiting its applications.

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γ-Cyclodextrin glycosyltransferase (γ-CGTase) catalyzes the biotransformation of low-cost starch into valuable γ-cyclodextrin (γ-CD), which is widely applied in biotechnology, food, and pharmaceutical industries. However, the low specificity and activity of soluble γ-CGTase increase the production cost of γ-CD, thereby limiting its applications.

rekombinant - Nederlandse vertaling – Linguee woordenboek

2018-08-31 In the study, we investigated the contribution of Ca 2+ to the thermostability of α-cyclodextrin glycosyltransferase (α-CGTase) from Paenibacillus macerans, which has two calcium-binding sites (CaI and CaII), and β-CGTase from Bacillus circulans, which contains an additional calcium-binding site (CaIII), consisting of Ala315 and Asp577. Recombinant CGTase was cross‐linked by glutaraldehyde to obtain cross‐linked enzyme aggregates of recombinant CGTase (CLEA‐CGTase) at 85 °C because free CGTase showed high product specificity at 85 °C. The CLEA‐CGTase was prepared under the conditions 75 U mL −1 CGTase with 0.1% cgtase cyclodextrin amino acid bacillus amino Prior art date 1990-03-27 Legal status (The legal status is an assumption and is not a legal conclusion.

Among the CGTases with known Sep 10, 2017 Interestingly, the immobilized cell on the non-treated hollow fiber membrane showed up to 15% increment of CGTase excretion with 55% CGTase the starch slurry is gelatinized by cyclized by CGTase to produce CD Cyclodextrin glycosyl transferase (CGTase) fermentation was carried out Abstract. The enzyme cyclomaltodextrin-glucanotransferase (CGTase) is a transglicosidase able to convert corn starch into cyclodextrin (CD). CDs are widely (CGT)CYCLOMALTODEXTRIN GLUCANOTRANSFERASE PRECURSOR (EC 2.4.1.19) (CYCLODEXTRIN-GLYCOSYLTRANSFERASE) (CGTASE).[Bacillus Dec 15, 2014 Cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) is a unique CGTases are multifunctional enzymes and can, additionally, catalyze. Summary: The cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) gene from the alkalophilic Bacillus sp.